Composite

Part:BBa_K5107007:Design

Designed by: Georgios Retsinias   Group: iGEM24_DTU-Denmark   (2024-09-22)


T7-HREmin-sB-T


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 72
    Illegal PstI site found at 60
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 60
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 72
    Illegal PstI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 72
    Illegal PstI site found at 60
    Illegal AgeI site found at 112
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

G-block from IDT and then PCR amplification with proper primers.

References